Anti-CD62L mAbs

Cat. No.

MABL-1059

Name

Anti-CD62L mAbs

Clone No.

AFD- OX-85

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC

Species Reactivity

Rat

Basic Information

Specificity

OX-12 binds to rat antibody kappa light  chains - has preference for IgKa allotype.

Alternative Name

L-selectin; LECAM-1; Ly-22; MEL-14; CD62   antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1;   Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing   receptor; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen

UniProt

P30836

Immunogen

This antibody was prepared by immunizing 8   –12-week-old BALB/c mice with purified rat CD62L-CD4

Application Notes

This antibody binds to CD62L expressed on   rat lymphocytes, and has been used to show that recombinant soluble rat   CD62L-CD4, to which it also binds, is likely to be correctly folded   (Nicholson, 1998). This antibody also labels, and can therefore be used to   fractionate, the subpopulation of rat CD4+CD8- thymocytes that expresess   L-selectin (Seddon, 1996). Cells with this phenotype are able to protect rats   from diabetes upon adoptive transfer, as well as providing B cells with help   for secondary antibody responses and mediating local graft-versus-host   reactions.

Antibody First Published

Seddon et al. CD4+CD8- thymocytes that express   L-selectin protect rats from diabetes upon adoptive transfer European Journal   of Immunology, Volume 26, Issue 11, 1996, Pages 2702-2708 PMID:8921958

Note on publication

Describes the original use of this antibody   to isolate the subpopulation of rat CD4+CD8- thymocytes that express   L-selectin.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.