Cat. No.
MABL-3154
Application
crystallography, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
anti-TDRKH-RAB-C143
From
Recombinant Antibody
Specificity
This antibody is specific for human Tudor and KH domain-containing protein.
Alternative Names
TDRKH; TDRD2; Tudor domain-containing protein 2
UniProt
Q9Y2W6
Immunogen
The original antibody was raised by panning against a phage-display library using recombinant TDRD2 eTudor.
Application Notes
The original antibody was used for an ELISA on recombinant TDRD2 eTudor. The structure of the original antibody was determined using x-ray crystallography. This antibody was used for a pull-down assay on TDRD2 eTudor domain (Chen et al., 2020; PMID:31288074). This antibody was used for immunofluorescence on different human cell lines. This antibody showed staining of the cytoplasm for all cell lines tested (Hornsby et al., 2015; PMID:26290498).
Antibody First Published
Hornsby et al. A High Through-put Platform for Recombinant Antibodies to Folded Proteins Mol Cell Proteomics. 2015 Oct;14(10):2833-47. PMID:26290498
Note on publication
The original paper describes an industrialized platform to generate antigens and validated recombinant antibodies for 346 transcription factors (TFs) and 211 epigenetic antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
