Cat. No.
MABL-3155
Application
EIA, FACS, TLC, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
GMA1
From
Recombinant Antibody
Specificity
This antibody is specific for the tumor-associated gangliosides GD3, GD2, and GM3. It showed minimal or no activity against GD1a, GD1b, GT1b, and GQ1b.
Alternative Names
GD2; GD3; GM3
UniProt
Immunogen
The original antibody was developed by fusing pooled lymph node lymphocytes from cancer patients with the human lymphoblastoid cell line, SHFP-1.
Application Notes
The specificity of the original antibody (human IgG1) was evaluated using ELISA and TLC. It exhibited high specificity for major tumor-associated gangliosides GD3, GD2, and GM3, with minimal cross-reactivity observed with other gangliosides such as GD1b, GT1b, and GQ1b. GD3 demonstrated the highest binding affinity, while GM3 exhibited the lowest. Additionally, the antibody showed binding to melanoma (SK-MEL-28), stomach (KATO-III), pancreas (PANC-1), and neuroblastoma (U87MG) cell lines in an enzyme immunoassay (EIA). It reacted with both live and fixed SK-MEL-28 and PANC-1 cells in IF and FACS assays, indicating its ability to bind both cell surface and intracellular antigens. A recombinant version of the antibody was developed and shown to have similar reaction characteristics as the antibody produced by the hybridoma cell line, demonstrating that the specificity was not altered during molecular cloning (EP1056861A2) (Mukerjee et al., 1998; PMID: 9627053).
Antibody First Published
Mukerjee et al., Characterization of human IgG1 monoclonal antibody against gangliosides expressed on tumor cells. Hybridoma. 1998 Apr;17(2):133-42. doi: 10.1089/hyb.1998.17.133. PMID:9627053
Note on publication
The original publication describes the development, characterization, and recombinant expression of a human IgG1 monoclonal antibody (GMA1) with specificity for tumor-associated gangliosides, demonstrating its selective reactivity with melanoma and neuroblastoma cells but not with normal tissues or other tumor types.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
