Cat. No.
MABL-3156
Application
functional assays, in vitro, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
PolrecCHOIgM/GD3
From
Recombinant Antibody
Specificity
This antibody is specific for human GD3, GM1, GD2, and GM3 gangliosides.
Alternative Names
GD3; GM3; GD2; GM1
UniProt
Immunogen
The original antibody was developed by isolating heavy and light chain cDNAs from a hybridoma producing an anti-GD3 IgM antibody. These sequences were then codon-optimized and transfected into CHO cells to produce the recombinant PolrecCHOIgM/GD3 antibody.
Application Notes
The original antibody (recombinant human IgM) was designed to bind the ganglioside GD3, which is overexpressed in malignant cells like melanomas. ELISA testing revealed strong binding specificity to GD3, GM1, GD2, and GM3 gangliosides, even at low concentrations. In cell-line assays, it selectively bound cancer cell lines (melanoma, lung, breast, and glioblastoma), without binding to normal cells such as huVEC and lung fibroblasts. Immunoimaging confirmed this selective binding, with fluorescence indicating strong interaction with malignant cells. In vitro complement activation tests showed that the antibody triggered cancer cell lysis when exposed to normal human serum, demonstrating its ability to activate the complement cascade. The antibody thus effectively discriminates between malignant and normal cells, confirming its diagnostic and therapeutic utility in cancer (US10590204B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
