Cat. No.
MABL-3162
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Leishmania donovani
Clone No.
NbA
From
Recombinant Antibody
Specificity
This antibody is specific for Leishmania donovani tyrosyl-tRNA synthetase.
Alternative Names
LdTyrRS; Leishmania donovani tyrosyl-tRNA synthetase
UniProt
Immunogen
The original antibody was generated by immunizing one llama (Lama glama) six times with in total 0.9 mg of LdTyrRS. Four days after the final boost, blood was taken to isolate peripheral blood lymphocytes.
Application Notes
The specificity of this antibody's original format was confirmed by ELISA analysis.
Antibody First Published
Barros-Álvarez et al. Leishmania donovani tyrosyl-tRNA synthetase structure in complex with a tyrosyl adenylate analog and comparisons with human and protozoan counterparts Biochimie. 2017 Jul;138:124-136. doi: 10.1016/j.biochi.2017.04.006 PMID:28427904
Note on publication
The original publication focuses on the crystal structure of Leishmania donovani tyrosyl-tRNA synthetase (LdTyrRS) complexed with a nanobody and a tyrosyl adenylate analog, highlighting the flexibility of LdTyrRS, the interactions of the analog with the active site, and the presence of an "extra pocket" that can guide the development of selective drugs for treating infections caused by L. donovani and other pathogenic protozoa.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
