Cat. No.
MABL-3172
Application
WB, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human
Clone No.
TE5
From
Recombinant Antibody
Specificity
This antibody is specific for an unidentified Golgi matrix protein.
Alternative Names
UniProt
Immunogen
The original antibody was generated via phage display from the Griffin.1 library by screening against Rat liver Golgi (RLG) complexes derived from rat liver.
Application Notes
The original format of this antibody (scFv) was successfully used in a Western blot and for in vitro immunofluorescence staining of the Golgi matrix in HeLa cells (Nizak et al., 2003; PMID: 14617357).
Antibody First Published
Nizak et al. Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo Traffic. 2003 Nov;4(11):739-53. doi: 10.1034/j.1600- 0854.2003.00132.x PMID:14617357
Note on publication
The original publication demonstrates the use of recombinant antibodies selected through phage display against subcellular fractions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
