Cat. No.
MABL-3173
Application
Isotype
Engineer antibody
Species Reactivity
Clone No.
NISTmab (RM 8671)
From
Recombinant Antibody
Specificity
Alternative Names
mAb 8671; RM8671; NIST8671; NISTmab1; reference material 8671
UniProt
Immunogen
Application Notes
This antibody is a recombinant version of NISTmAb, a humanized IgG1k reference material 8671 (RM 8671) from the National Institute of Standards and Technology. It is a widely characterized monoclonal antibody, intended for use in evaluating the performance of methods for determining physicochemical and biophysical attributes of monoclonal antibodies. This antibody acts as a representative test molecule for development of novel technology for therapeutic protein characterization. It is intended for a variety of uses that may include system suitability tests, establishing method or instrument performance and variability, comparing changing analytical test methods, and assisting in method qualification (Reference Material 8671 information sheet). This antibody can be used as a standard for the benchmarking of top-down mass spectrometry for analysis of intact proteins (PMID: 31249407). It was also used as a standard for de novo sequencing algorithm on bottom-up mass spectrometry (PMID: 28105549). It was also used as a standard for calibration of a technique for estimating the monomeric purity and antigen affinity of research grade antibody samples (PMID: 31375854). Note: *NIST is a registered trademark owned by National Institute of Standards and Technology
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
