Cat. No.
MABL-3174
Application
inhibit, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Helicobacter pylori
Clone No.
HpU-2
From
Recombinant Antibody
Specificity
HpU-2 binds specifically to H. pylori at the epitope with sequence SVELIDIGGNRRIFGFNALVDR - the sequence is part of a loop near to the C-terminus of the alpha subunit of urease (the beta subunit possesses the active site). HpU-18 competes with HpU-2 for binding to this epitope. H. pylori is a gram negative spiral bacterium and causes numerous gastroduodenal diseases. It expresses large amounts of urease when present in the human stomach; urease is key for the survival and pathogenesis of the bacteria as it neutralizes the acidity in the stomach by hydrolysis of urea into ammonia.
Alternative Names
urease-alpha; alpha-urease; urease
UniProt
P14916
Immunogen
HpU-2 was prepared by subcutaneous injection of purified H. pylori urease into BALB/c mice.
Application Notes
HpU-2 results in ~82% inhibition (allosteric inhibition) of urease activity and the inhibitory activity is increased to 87% when HpU-2 is used in combination with HpU-19. HpU-2 has been shown be unsuccessful for use in IHC of formalin-fixed sections of H. pylori-infected human cells. HpU-2 can also be used in SPR and ELISA studies.
Antibody First Published
Fujii et al. Epitope mapping and features of the epitope for monoclonal antibodies inhibiting enzymatic activity of Helicobacter pylori urease. Biotechnol Bioeng. 2004 May 20;86(4):434-44. PMID:15112296
Note on publication
Describes the investigation into the inhibitory mechanism of HpU-2 against H. pylori urease. The binding epitope was determined by SPR and molecular modelling.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
