Cat. No.
MABL-3181
Application
WB, Block, ELISA
Isotype
Engineer antibody
Species Reactivity
G. vaginalis
Clone No.
9B4
From
Recombinant Antibody
Specificity
The antibody binds to VLY with an affinity (Kd) of 0.29 nM and an IC50 of 0.067 nM (determined by hemolytic assay). The antibody has a strong neutralizing capability.
Alternative Names
VLY
UniProt
Immunogen
Recombinant VLY.
Application Notes
The antibody binds specifically to VLY, a pore-forming toxic member of the cholesterol- dependent cytolysins, produced by the bacterium Gardnerella vaginalis. Once released, VLY acts by binding to cholesterol-rich membranes and forming transmembrane pores that result in lysis of the cell. The bacterium G. vaginalis is predominant in the vaginal tract of women with bacterial vaginosis (BV). Here releases VLY toxins that cause cytolysis and tissue destruction, and potentially leading to serious adverse effects such as infertility. Antibodies that are able to neutralize the toxic activity of VLY can be used as therapeutic agents for the treatment of BV. The antibody 9B4 has the strongest neutralizing activity, and binds to an epitope region comprising of the amino acids 122 to 268 of VLY.
Antibody First Published
Zvirbliene A, Pleckaityte M, Lasickiene R, Kucinskaite-Kodze I, Zvirblis G. Production and characterization of monoclonal antibodies against vaginolysin: mapping of a region critical for its cytolytic activity. Toxicon. 2010 Aug 1;56(1):19-28. PMID:20298711
Note on publication
Describes the generation of two monoclonal antibodies with neutralizing activity against vaginolysin, the causative agent of bacterial vaginosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
