Cat. No.
MABL-3182
Application
crystallization, SPR, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Trypanosoma
Clone No.
cAbAn33
From
Recombinant Antibody
Specificity
The antibody is specific for the conserved carbohydrate moiety on variant surface glycoprotein of the trypanosome coat. The antibody cross-reacts with other VSGs (AnTat 1.1, MITat 1.1, MITat 1.5, and deglycosilated AnTat 1.1).
Alternative Names
VSG; cAb-An33; cAbAn33_1-0-0; VSG 221; Variant surface glycoprotein MITAT 1.2
UniProt
P26332
Immunogen
The antibody was generated by immunizing a dromedary (Camelus dromedarius) with VSG purified from T. brucei brucei AnTat 1.1. A VHH library was constructed and the antibody was isolated by panning against immobilized AnTat 1.1.
Application Notes
The antibody specificity and possible cross-reactivity with other VSGs (AnTat 1.1, MITat 1.1, MITat 1.5, and deglycosilated AnTat 1.1) was analyzed via solid-phase ELISA. The antibody recognized all the VSGs. The capacity of cAb-An33 to recognize its epitope on living trypanosomes (AnTat 1.1, MITat 1.1, or MITat 1.5) was analyzed via flow cytometry and microscopy. Flow cytometry showed that the antibody stains parasites of the three VSG classes, although not to the same intensity. Finally, the antibody was fused together with beta-lactamase and employed as a recognition unit in immuno-toxins for trypanosomosis therapy (Stijlemans et al., 2004; PMID: 14527957). The crystal structure of the VHH fragment was determined. The humanized version of the antibody was constructed and the crystal structure determined. The binding affinity of the VHH and the humanized fragments to VSG was measured by surface plasmon resonance (Kd= 28 nM and 539.5 nM respectively) (Conrath et al., 2005; PMID:15913651).
Antibody First Published
Stijlemans et al. Efficient targeting of conserved cryptic epitopes of infectious agents by single domain antibodies. African trypanosomes as paradigm J Biol Chem. 2004 Jan 9;279(2):1256-61. PMID:14527957
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
