Cat. No.
MABL-3183
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1E 9
From
Recombinant Antibody
Specificity
This antibody is specific for the C-terminal domain of human VASA protein. The binding epitope comprises amino acids 712-721 (SQAPNPVDDE) of the human VASA protein. VASA homolog is also known as ATP-dependent RNA helicase DDX4, which is required during spermatogenesis. VASA protein is present in fetal and adult gonadal germ cells in both males and females and is most abundant in spermatocytes and mature oocytes.
Alternative Names
DDX4; VASA; VASA-1; Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog; 1E9/VH1E3
UniProt
Q9NQI0
Immunogen
The original antibody was isolated from an scFv phage display library generated from the pool of normal human B cells from 40 blood donors. The particular antibody was isolated by panning the library against human VASA-1 peptide (amino acids 712-721). Later on the scFv antibody was converted to an IgG.
Application Notes
The binding characterization of this antibody to human VASA protein and VASA peptides (residues 700-724; residues 712-721; residues 700-709) was done using ELISA. It was found that this antibody was specific for VASA peptide with residues 712-721. The IgG antibody binds human VASA protein in a dose dependent manner with an EC50 of 0.08919 nM (US20180155445).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
