Cat. No.
MABL-3187
Application
WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HAE-2z
From
Recombinant Antibody
Specificity
This antibody is specific for human VCAM-1.
Alternative Names
VCAM-1; V-CAM 1; INCAM-100; CD106
UniProt
P19320
Immunogen
The original antibody was raised by immunizing a mouse with domain 1 of human VCAM-1.
Application Notes
The original antibody was used for immunofluorescence on HCAEC. It was able to show the localization of VCAM-1. This antibody was used for a western blot on extracts of HCAEC (Seye et al., 2003; PMID:12714597), and on mesenteric arteries derived from WT and A2BAR-KO mice (Yang et al., 2006; PMID:16823489), and on Mouse microvascular EC (Zerr et al., 2011; PMID:21576651). This antibody was used for an ELISA on HUVECs to show VCAM-1 (Haguet et al., 2020; PMID:32719607). The effect of this antibody on the growth of human blood outgrowth endothelial cells in mice was tested (Milbauer et al., 2009; PMID:19304277).
Antibody First Published
Seye et al. The P2Y2 nucleotide receptor mediates UTP-induced vascular cell adhesion molecule-1 expression in coronary artery endothelial cells. J Biol Chem. 2003 Jul 4;278(27):24960-5. PMID:12714597
Note on publication
In the original paper they addressed the hypothesis that activation of P2Y2 receptors by extracellular nucleotides modulates the expression of adhesion molecules on vascular endothelial cells that are important for monocyte recruitment.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
