Cat. No.
MABL-3207
Application
inhibition, ELISA
Isotype
Engineer antibody
Species Reactivity
Clone No.
NbVIM_38
From
Recombinant Antibody
Specificity
This antibody is specific for VIM-4 M beta L. The main epitope of this antibody is amino acid 99 to 133.
Alternative Names
UniProt
Immunogen
The original antibody was raised by panning a dromedary phage display library against VIM-4 M beta L.
Application Notes
The epitope of this antibody was determined using ELISA on different parts of its antigen. The ability of this antibody to inhibit VIM-4 was shown. The presence of this antibody inhibited activity of VIM-4 against imipenem, benzylpenicillin and cephalothin (Sohier et al., 2013; PMID:23289540).
Antibody First Published
Sohier et al. Allosteric inhibition of VIM metallo-β-lactamases by a camelid nanobody Biochem J. 2013 Mar 15;450(3):477-86. PMID:23289540
Note on publication
The original paper used an approach on the basis of phage display was used to select single-domain antibody fragments (VHHs, also called nanobodies) that would inhibit the clinically relevant VIM (Verona integron-encoded MβL)-4 MβL.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
