Cat. No.
MABL-3208
Application
IF
Isotype
Engineer antibody
Species Reactivity
Human, dog, rat
Clone No.
1433
From
Recombinant Antibody
Specificity
This antibody binds the vimentin intermediate filament. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Alternative Names
VIM
UniProt
P08670
Immunogen
The original antibody was generated from an immunized alpaca.
Application Notes
This chimeric engineered antibody is generated by fusing the original alpaca derived nanobody to mouse IgG1 and rabbit Fc domains to extend the research application of the original. This antibody binds vimentin present on canine MDCK cells, rodent BHK cells and on human A549 cells upon stimulation with TGFβ. This antibody can be used for immunofluorescent staining of tissues expressing vimentin. It can also be conjugated with fluorophores for direct detection. It can also be immobilized on protein A, beads, resins and plates. The original alpaca VHH antibody is available from ChromoTek.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
