Cat. No.
MABL-3210
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Nb79
From
Recombinant Antibody
Specificity
This antibody binds the human vimentin. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Alternative Names
VIM
UniProt
P08670
Immunogen
The original antibody was isolated from a VHH library generated by immunization of an alpaca with whole glioblastoma multiforme (GBM) cells.
Application Notes
The binding reactivity of this antibody towards vimentin was checked using ELISA. This antibody can also be used to identify vimentin in a western blot (PMID: 28498803).
Antibody First Published
Jovčevska et al. Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates. Oncotarget. 2017 Jul 4;8(27):44141-44158. PMID:28498803
Note on publication
Describes the isolation of nanobodies against various antigens overexpressed in glioblastoma multiforme.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
