Cat. No.
MABL-3214
Application
ICC, IEM, IHC-P, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
YT33
From
Recombinant Antibody
Specificity
This antibody specifically recognizes vimentin phosphorylation at Ser33, a C kinase phosphorylation site.
Alternative Names
UniProt
P31000
Immunogen
The original antibody was generated by immunizing BDF1 mice with keyhole limpet hemocyanin (KLH) coupled PV33 — a synthesized vimentin peptide containing the ser33 phosphorylation — (PV33-KLH) emulsified in complete Freund's adjuvant.
Application Notes
This antibody is specific to the C kinase phosphorylated vimentin Ser33 residue, as determined by ELISA, can detect the activity of endogenous CAM kinase II astrocytes, as detected by WB, and was used for ICC (IF) to detect C kinase phosphorylated vimentin Ser33 in type 1 astrocytes extracted from newborn rats' cerebral cortices (Ogawara et al., 1995; PMID: 7490282). This antibody was used for: in vivo vimentin kinase activity visualization of vimentin kinase activity in cell signaling or mitosis (Goto et al., 1998; PMID: 9565595), IHC-p and IF staining of human glioma cells (Fujita et al., 2004; PMID: 15597762), and as a positive control in a WB for vimentin ser55 phosphorylation (Yamaguchi et al., 2005; PMID: 16260496).
Antibody First Published
Ogawara et al. Differential targeting of protein kinase C and CaM kinase II signalings to vimentin J Cell Biol. 1995 Nov; 131(4):1055-66. doi: 10.1083/jcb.131.4.1055 PMID:7490282
Note on publication
The original publication prepared YT33 to investigate the signaling pathways of C kinase to vimentin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
