Cat. No.
MABL-3216
Application
immunoblot, in vitro, in vivo, PNT, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
VHSV, Viral hemorrhagic septicemia virus
Clone No.
3F1H10
From
Recombinant Antibody
Specificity
This antibody is specific for the transmembrane envelope glycoprotein (G protein) of viral hemorrhagic septicemia virus (VHSV). The G protein is the only protein known to be present on the surface of the virus particle, and it constitutes less than 10% of the mass of the virus particle which is dominated by N, M1 and M2.
Alternative Names
G; Spike glycoprotein; MAb-I
UniProt
P27662
Immunogen
The original antibody was generated by immunizing BALB/c mice with Egtved virus by intraperitoneal injections five times over two months, followed by an additional intravenous booster injection on week nine. The hybridoma cell line secreting clone 3F1H10 was selected from a subsequent fusion after three more months of continued immunization.
Application Notes
The neutralizing activity of the original format of this antibody (mouse IgG1) was measured at the cell culture level in a plaque neutralization test (50% PNT). This antibody was administered to rainbow trout via intraperitoneal injection to evaluate its passive immunization protective effect and efficacy against Egtved virus infection. This antibody was used as the primary antibody in immunoblotting (IB) to detect the viral glycoprotein. This antibody, conjugated with fluorochromes like FITC or TRITC, was employed to visualize and detect Egtved virus-infected cells. This antibody, which was highly neutralizing in vitro, also provided the highest degree of protection in vivo of the antibodies tested (Lorenzen et al., 1990; PMID: 1690259). This antibody's neutralizing activity against different VHSV isolates was determined by 50% PNT; This antibody's original format neutralized type I isolates VHSV (I-F1 and I-92) well, but higher concentrations were needed to neutralize type II isolates and even higher concentrations were required to neutralize type III isolates.
Antibody First Published
Lorenzen et al. Neutralization of Egtved virus pathogenicity to cell cultures and fish by monoclonal antibodies to the viral G protein J Gen Virol. 1990 Mar;71 ( Pt 3):561-7. doi: 10.1099/0022-1317-71-3-561 PMID:1690259
Note on publication
The original publication focuses on the characterization of monoclonal antibodies to the viral G protein of Egtved virus, demonstrating their neutralizing activity in vitro and their protective effects in rainbow trout.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
