Key features and details | |
Cat. No. | MABL-1106 |
Name | Anti-CD79a mAbs |
Clone No. | AFD- HM57 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, IF, IHC |
Species Reactivity | Chicken, Cow, Guinea Pig, Horse, Opossum, Pig, Primate, Rabbit, Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody recognises CD79a which is involved in initiation of the signal transduction cascade activated by binding of antigen to the B-cell receptor. CD79a is used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissue sections. |
Alternative Name | MB-1; B-cell antigen receptor complex-associated protein alpha chain; Ig- alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM- associated protein; MB-1 membrane glycoprotein |
UniProt | P11912 |
Immunogen | This antibody was raised by immunising BALB/c mice with a synthetic peptide G-T-Y-Q-D-V-G- S-L-N-I-A-D-V-Q, representing amino acids 202-216 of the human mb-1 protein and conjugated to thyroglobulin. |
Application Notes | CD79a is strongly expressed in various B-cell-derived conditions and thus this antibody is useful during identification and investigation of such diseases (via IF, IHC) (Mason et al., 1991). One group utilised it for immunofluorescent labelling of CD79a containing cells in bone marrow samples of pre-B lymphocyte acute leukemia patients (Mason et al., 1991). HM57 antibody can also be used for IP as it was very efficient in immunoprecipitating CD79a from NP-40 cells lysates (Mason et al., 1995). Finally, this antibody has been widely applied in veterinary research and proved successfull in recognising CD79a in various species, from chicken to dog (Doring et al., 2018). |
Antibody First Published | van Noesel et al. The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene. J Immunol. 1991 Jun 1;146(11):3881-8. PMID:2033258 |
Note on publication | This article describes generation and application of HM57 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
