Anti-CD8 mAbs

Cat. No.

MABL-1119

Name

Anti-CD8 mAbs

Clone No.

AFD- G10-1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

immunotoxin, IP, FC, IF, IHC

Species Reactivity

Macaca nemestrina, Human

Basic Information

Specificity

This antibody is specific for human CD8, and   binds human CD8+ T cells. It also cross-reacts with non-human primate CD8,   including that of Macaca nemestrina (Southern pig-tailed macaque).

Alternative Name

cluster of differentiation 8; G10.1; Tp32

UniProt

Immunogen

Human CD8.

Application Notes

This antibody has been used to stain cells expressing   CD8 by indirect immunofluorescence (Uckun et al, 1989) and in   immunohistochemical analysis of aortic and iliac artery tissue from nonhuman   primates (Masuda & Ross, 1989). It has also been employed in   immunoprecipitation and gel electrophoresis experiments to investigate cell   surface CD8 complexes (Ledbetter et al, 1985). A FITC-conjugated version of   this antibody has been used to identify T cell populations in flow cytometry   experiments (Chen et al, 2003). When conjugated to the ribosome-inhibitory   protein ricin, this antibody acts as a potent CD8+ T cell-directed   immunotoxin (Uckun et al, 1989).

Antibody First Published

Ledbetter et al. Covalent association   between human thymus leukaemia-like antigens and CD8(Tp32) molecules. J   Immunol. 1985 Jun;134(6):4250-4 PMID:3921621

Note on publication

Describes the use of this antibody in   immunoprecipitation and gel electrophoresis experiments

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.