Key features and details | |
Cat. No. | MABL-1120 |
Name | Anti-CD8 mAbs |
Clone No. | AFD- OKT8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the CD8 gene in humans. CD8 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, it functions primarily as a coreceptor for MHC class I molecule |
Alternative Name | T-cell surface glycoprotein CD8 alpha chain; CD8A; T-lymphocyte differentiation antigen T8/Leu-2 |
UniProt | P01732 |
Immunogen | This antibody was raised by immunizing CAF mice with human thymocytes. |
Application Notes | CD8+ T cell effector function assays were preformed using this antibody. The cell lines used for this study are HLA A*0201-restricted CD8+ T cell clones: 1) ILA1, specific for the human telomerase reverse transcriptase-derived epitope ILAKFLHWL (residues 540–548) (30, 31); 2) ALF3, specific for the influenza A matrix protein (M1)-derived epitope GILGFVFTL (residues 58–66); and 3) MEL5 and MEL187.c5, specific for the Melan-A–derived epitope ELAGIGILTV (residues 26–35) (32). The HLA B*0801-restricted CD8+ T cell clone LC13 is specific for the EBV EBNA3A-derived epitope FLRGRAYGL (residues 339–347) (33), and the HLA B*3508-restricted CD8+ T cell clone SB10 is specific for the EBV BZLF1-derived epitope LPEPLPQGQLTAY (residues 52–64) (34). The HLA DR*0101-restricted CD4+ T cell clone C6 recognizes the influenza A hemagglutinin-derived epitope PKYVKQNTLKLAT (residues 307–319). CD8+ T cell lines specific for the EBV BMLF1-derived epitope GLCTLVAML (residues 280–288). (Clement et al, 2011; pmid:21677135). |
Antibody First Published | Reinherz et al. Discrete stages of human intrathymic differentiation: Analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage Proc Natl Acad Sci U S A. 1980 Mar;77(3):1588-92. PMID:6966400 |
Note on publication | A series of monoclonal antibodies was used to define three discrete stages of human intrathymic T-cell differentiation. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
