Anti-CXCR3 mAbs

Cat. No.

MABL-1303

Name

Anti-CXCR3 mAbs

Clone No.

AFD- 9C5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FC, ELISA

Species Reactivity

Marmoset, Human, Mouse

Basic Information

Specificity

This antibody strongly binds human, mouse   and marmoset CXCR3. Epitope mapping revealed that this antibody binds a   peptide (FSSSYDYGENESDSCCTSPPCP) which corresponds to amino acid position   23-44 of the human CXCR3.

Alternative Name

CD183; CXC-R3; CXCR-3; CKR-L2; IP-10 receptor; GPR9; C-X-C chemokine receptor type 3; G protein-coupled receptor   9; Interferon-inducible protein 10 receptor

UniProt

P49682

Immunogen

The original antibody was generated by immunizing   a CXCR3* knockout mice with LI.2 cells (a murine pre-B cell line) were   transfected with the mouse CXCR3 gene.

Application Notes

The binding of this antibody with CXCR3 from   various species was assessed by flow cytometry. It was shown to recognize   LI.2 cells transfected with mouse CXCR3 (mCXCR3) or human CXCR3 (hCXCR3), but   not untransfected LI.2 cells or cells transfected with other chemokine   receptors (i.e. mCXCR2) in flow cytometry. Epitope mapping of this antibody   was performed using ELISA. To access the in vivo effect, various   concentrations of the antibody were injected intraveneously in a mice. It was   seen that the original 9C4, mouse IgG1 version, completely depleted   CD4+CXCR3+, CD8+/CXCR3+ and B2207CXCR3+ cells at a concentration as low as 2   mg/kg (WO2014186842). Chimeric antibodies derived from 9C5 called 9C5-3MFc   and 9C5-Fc-KO and humanized verion are also available on request.

Antibody First Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.