Key features and details | |
Cat. No. | MABL-1304 |
Name | Anti-CXCR3 mAbs |
Clone No. | AFD- CXCR3-173 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, Chemotaxis Assay, FACS, in vivo, inhibition, LBA, therapeutic, ELISA, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for mouse CXCR3 and recognizes CXCR3 on cells from wild-type but not CXCR3-/- mice. |
Alternative Name | CD183; Mouse CXCR3; CXC-R3; CXCR-3; C-X-C chemokine receptor type 3; Interferon-inducible protein 10 receptor; IP-10 receptor |
UniProt | O88410 |
Immunogen | The original antibody was generated by immunizing Armenian hamsters with a multiple antigen peptide (MAPS8) encompassing amino acids 1-37 of mouse CXCR3 in complete Freund's adjuvant, and boosted with peptide in incomplete Freund's adjuvant. |
Application Notes | This antibody’s original format (hamster (Armenian) IgG) was used in ligand binding assay (LBA), FACS-based chemokine binding inhibition assay, and chemotaxis assays, as well as to measure its pharmacokinetics and immunogenicity in a mouse model. It blocked chemotaxis in response to CXCL10 or CXCL11 in vitro. When injected into mice, this antibody significantly prolonged cardiac and islet allograft survival. When combined with a subtherapeutic regimen of rapamycin, this antibody induced long-term (>100 d) survival of cardiac and islet allografts (Uppaluri et al., 2008; PMID: 18622291). This antibody was used to block CXCR3 to investigate its role in lymphocyte migration in vivo. The antibody was given intravenously just before an injection of labeled T cells to assess the effect of blocking CXCR3 on T cell migration (Dai et al., 2016; PMID: 27412416). |
Antibody First Published | Uppaluri et al. Prolongation of cardiac and islet allograft survival by a blocking hamster anti-mouse CXCR3 monoclonal antibody Transplantation. 2008 Jul 15;86(1):137-47. doi: 10.1097/TP.0b013e31817b8e4b PMID: 18622291 |
Note on publication | The original publication reports the generation, characterization, and use of CXCR3- 173. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
