Anti-IL-6R mAbs

Cat. No.

MABL-2104

Name

Anti-IL-6R mAbs

Clone No.

AFD- D7715A7

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, therapeutic, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine IL-6R.

Alternative Name

CD126; 15A7; IL-6Ra; IL6R; Interleukin-6   receptor subunit alpha; Il6ra; IL- 6 receptor subunit alpha; IL-6R subunit   alpha; IL-6R-alpha; IL-6RA; IL-6R 1

UniProt

P22272

Immunogen

This antibody was raised by immunising Lewis   rats with OKT4 cells.

Application Notes

This antibody binds to IL-6R-positive cells,   and blocks IL-6 binding (Vink et al, 1990; Coulie et al, 1990). This antibody  is able to inhibit the growth of IL6-dependent plasmacytoma T1033C2 in vitro   (Coulie et al, 1990). Administration of this antibody to mice prior to   challenge with T1033C2 resulted in a protection rate of 60% and prolonged   survival in tumour-bearing mice (Vink et al, 1990). This antibody has been   used in flow cytometry to assess IL-6Ra expression (Hoge et al, 2013). When   added to dendritic cells subject to CD40 activation in vitro, this antibody   neutralises IL-6 and ablates the effect of CD40 activation (Grohmann et al,   2001).

Antibody First   Published

Coulie et al. A monoclonal antibody specific  for the murine IL-6-receptor inhibits the growth of a mouse plasmacytoma in vivo. Curr Top Microbiol Immunol. 1990;166:43-6. PMID:2073815

Note on publication

Describes the original generation of this antibody, and the characterisation of its ability to block IL-6 binding.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.