Key features and details | |
Cat. No. | MABL-2108 |
Name | Anti-IL-8 mAbs |
Clone No. | AFD- 6G4.2.5V11N35A |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize, ELISA |
Species Reactivity | Rabbit, Human, Rhesus Monkey |
Basic Information | |
Specificity | This antibody binds human IL-8, which is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. This antibody cross reacts with rabbit and rhesus monkey IL-8. |
Alternative Name | IL8; Interleukin-8; CXCL8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor; 6G4.2.5; V11N35A; 6G4V11N35A |
UniProt | P10145 |
Immunogen | The original mouse antibody 6G4.2.5 was generated by immunizing BALB/c mice with recombinant human IL-8. The humanized version is 6G4.2.5 V11N35A was generated by grafting CDRs of mouse parental antibody onto human framework regions. |
Application Notes | This antibody is capable of neutralizing the biological activity of wild-type human IL-8. This antibody is reported to inhibit human wild type IL-8, human monomeric IL-8, rabbit IL-8, and rhesus IL-8 mediated neutrophil chemotaxis (US6025158). The binding characterization of this antibody was done using ELISA. The Fab version of this antibody binds human IL-8 with an affinity of 114 pM (US6025158). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
