Key features and details | |
Cat. No. | MABL-2297 |
Name | Anti-M protein, serotype 5 mAbs |
Clone No. | AFD- 24.1.2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Streptococcus pyogenes, Human |
Basic Information | |
Specificity | This antibody reacts with α-helical molecules and is anti-nuclear (binds DNA). Specifically, it binds streptococcal M-protein (type 5), human myosin, actin, tropomyosin, and troponin. |
Alternative Name | Myosin; Streptococcal M-protein; Actin; Tropomyosin; Troponin |
UniProt | P02977 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with streptococcal M type 5 membranes followed by a second, boosting dose of Lysin M5. |
Application Notes | This antibody binds myosin at a low relative avidity, as determined in a competitive ELISA (Mertens et al., 2000; PMID: 11282394). |
Antibody First Published | Cunningham et al. Human and murine antibodies cross-reactive with streptococcal M protein and myosin recognize the sequence GLN-LYS-SER-LYS-GLN in M protein J Immunol. 1989 Oct 15;143(8):2677-83 PMID:2677144 |
Note on publication | The original publication describes the use of overlapping synthetic peptides to map the myosin cross-reactive epitopes of streptococcal M protein recognized by antibodies from acute rheumatic fever and rheumatic heart disease sera. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
