Anti-M protein, serotype 5 mAbs

Cat. No.

MABL-2297

Name

Anti-M protein, serotype 5 mAbs

Clone No.

AFD- 24.1.2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Streptococcus pyogenes, Human

Basic Information

Specificity

This antibody reacts with α-helical   molecules and is anti-nuclear (binds DNA). Specifically, it binds   streptococcal M-protein (type 5), human myosin, actin, tropomyosin, and   troponin.

Alternative Name

Myosin; Streptococcal M-protein; Actin;   Tropomyosin; Troponin

UniProt

P02977

Immunogen

The original antibody was generated by immunizing BALB/c mice with streptococcal M type 5 membranes followed by a second, boosting dose of Lysin M5.

Application Notes

This antibody binds myosin at a low relative   avidity, as determined in a competitive ELISA (Mertens et al., 2000; PMID:  11282394).

Antibody First   Published

Cunningham et al. Human and murine   antibodies cross-reactive with streptococcal M protein and myosin recognize   the sequence GLN-LYS-SER-LYS-GLN in M protein J Immunol. 1989 Oct   15;143(8):2677-83 PMID:2677144

Note on publication

The original publication describes the use   of overlapping synthetic peptides to map the myosin cross-reactive epitopes   of streptococcal M protein recognized by antibodies from acute rheumatic   fever and rheumatic heart disease sera.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.