Key features and details | |
Cat. No. | MABL-2298 |
Name | Anti-M protein, serotype 5 mAbs |
Clone No. | AFD- 27.4.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | HA, ELISA, IF |
Species Reactivity | Streptococcus pyogenes, Human |
Basic Information | |
Specificity | This antibody reacts with α-helical molecules. Specifically, it binds streptococcal M-protein (type 5), human myosin, keratin, tropomyosin, ganglioside, and glycophorin M,N. |
Alternative Name | Myosin; Streptococcal M-protein; Keratin; Tropomyosin; Ganglioside; Glycophorin M,N |
UniProt | P02977 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with streptococcal M type 5 membranes followed by a second, boosting dose of Lysin M5. |
Application Notes | This antibody reacts with the human retina, as shown in an indirect immunofluorescence assay. This antibody reacts with retinal S-antigen (S-Ag), as shown in an ELISA (Lerner et al., 1995; PMID: 8722579). This antibody binds myosin at a high relative avidity, as determined in a competitive ELISA. Reactivity with glycophorin M and N was determined by hemagglutination assay (Mertens et al., 2000; PMID: 11282394). |
Antibody First Published | Cunningham et al. Human and murine antibodies cross-reactive with streptococcal M protein and myosin recognize the sequence GLN-LYS-SER-LYS-GLN in M protein J Immunol. 1989 Oct 15;143(8)：2677-83 PMID:2677144 |
Note on publication | The original publication describes the use of overlapping synthetic peptides to map the myosin cross-reactive epitopes of streptococcal M protein recognized by antibodies from acute rheumatic fever and rheumatic heart disease sera. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
