Key features and details | |
Cat. No. | MABL-2299 |
Name | Anti-M protein, serotype 5 mAbs |
Clone No. | AFD- 36.2.2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | HA, PRNT, WB, ELISA, IF |
Species Reactivity | Coxsackievirus, Poliovirus type 1, Rat, Streptococcus pyogenes, Human |
Basic Information | |
Specificity | This antibody reacts with α-helical molecules. Specifically, it binds streptococcal M-protein (type 5), human myosin, actin, keratin, tropomyosin, troponin, laminin, ganglioside, glycophorin M,N |
Alternative Name | Streptococcal M-protein; Myosin; Actin; Keratin; Tropomyosin; Troponin; Laminin; Ganglioside; Glycophorin M,N |
UniProt | P02977 |
Immunogen | The original antibody was generated by immunizing BALB/c BYJ mice with streptococcal M type 5 membranes followed by a second, boosting dose of M type 5 streptococcal membranes. |
Application Notes | This antibody reacts with human heart cells and whole streptococci (group A, M type 5), as shown in an ELISA, and human heart cells, human sarcolemma, and group A streptococcal membranes, as shown in a WB (Cunnigham et al., 1984; PMID: 6384047). This antibody reacts with the heavy chain of skeletal muscle myosin. Specifically, it reacts with light meromyosin fragments, as shown in WB and competitive inhibition assays (Cunningham et al., 1986; PMID: 3510006). This antibody labels the cytoplasm of epidermal cells and epidermal appendages in normal and psoriatic skin, as shown by immunofluorescent staining (Swerlick et al., 1986; PMID: 3525693). |
Antibody First Published | Cunningham et al. Murine monoclonal antibodies reactive with human heart and group A streptococcal membrane antigens Infect Immun. 1984 Oct;46(1):34-41. doi:10.1128/iai.46.1.34-41.1984 PMID:6384047 |
Note on publication | The original publication characterizes the antibody's reactivity with human heart streptococcal antigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
