Anti-Metarhodopsin-I mAbs

Cat. No.

MABL-2352

Name

Anti-Metarhodopsin-I mAbs

Clone No.

AFD- K42-IL

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Bovine

Basic Information

Specificity

The antibody binds specifically to the   C3-loop of metarhodpsin-I with a Kd of 2.8 nM, the epitope being TGALQERSK.

Alternative Name

UniProt

P02699

Immunogen

Bovine rod cell disc membranes.

Application Notes

This antibody specifically binds to the   C3-loop of metarhodopsin-I, which is in equilibrium with the meta-active   metarhodopsin II. The antibody stabilises the inactive metarhodopsin-I,   inhibiting G-protein activation.

Antibody First   Published

Adamus et al. Use of peptides to select for   anti-rhodopsin antibodies with desired amino acid sequence specificities.   Peptide Research 1988; 1(1):42-47 PMID:2485225

Note on publication

Describes the generation of several   monoclonal antibodies against rhodopsin using peptides for hybridoma   selection.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.