Key features and details | |
Cat. No. | MABL-2493 |
Name | Anti-Non muscle Myosin IIA mAbs |
Clone No. | AFD- SF9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF |
Species Reactivity | Drosophila, Rat, Human |
Basic Information | |
Specificity | This antibody is specific for a very small (29 aa long; 3 kDa) epitope of non muscle Myosin IIA, starting at aa 1536 in the full human sequence. |
Alternative Name | nmMyoIIA; NMMHC II-a; NMMHC-IIA; NMMHC-A; Non-muscle myosin heavy chain Iia; Myosin-9; Cellular myosin heavy chain, type A; Myosin heavy chain 9; Myosin heavy chain, non-muscle Iia; Non-muscle myosin heavy chain A |
UniProt | |
Immunogen | The original antibody was generated via phage display from the Griffin.1 library by screening against Rat liver Golgi (RLG) complexes derived from rat liver. |
Application Notes | The original format of this antibody (scFv) was successfully used in a Western blot and in for in vitro immunofluorescence staining of non muscle Myosin IIA (nmMyoIIA) in HeLa cells (Nizak et al., 2003; PMID: 14617357). Precise characterization of the SF9 target was achieved, defining a very small (3 kDa) epitope within the ~200kDa nmMyoIIA protein, revealing cross-reactivity with closely related proteins of the same and across species (Vielemeyer et al., 2010; PMID: 20727208). |
Antibody First Published | Nizak et al. Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo Traffic. 2003 Nov;4(11):739-53. doi: 10.1034/j.1600- 0854.2003.00132.x PMID:14617357 |
Note on publication | The original publication demonstrates the use of recombinant antibodies selected through phage display against subcellular fractions. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
