Anti-OPGbp mAbs

Cat. No.

MABL-2548

Name

Anti-OPGbp mAbs

Clone No.

AFD- AT

From

Recombinant Antibody

Isotype

Engineer antibody

Application

inhibition, SPR, ELISA

Species Reactivity

Human

Basic Information

Specificity

The antibody recognizes Human OPGbp   (residues 143-317). OPGbp acts as decoy receptor for TNFSF11/RANKL and   thereby neutralizes its function in osteoclastogenesis. It inhibits the   activation of osteoclasts and promotes osteoclast apoptosis in vitro.

Alternative Name

Osteoprotegerin binding protein; Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis   inhibitory factor; Osteoprotegerin

UniProt

O00300

Immunogen

The original antibody was generated by   panning against a human Fab library.

Application Notes

The specificity of the original format of the   antibody was confirmed by ELISA analysis. The binding affinity of the Fab and   IgG1 fragments of the antibody to OPGbp was measured by surface plasmon   resonance (Kd≈ 140 nM and 0.33-0.43 nM respectively). The interaction of   human OPGbp and its receptor ODAR was measured in a fluorescence energy   transfer (FRET) assay. The Fab fragment of the antibody inhibited OPGbp   binding to soluble ODAR-Fc fusion with an IC50 of 550 nM. Bone Marrow Assay   using the Fab fragment of the antibody gave a 50% decrease (IC50) at 57.8 nM.   In RAW Cell Assay the Fab fragment of the antibody showed an IC50 of 15   μg/ml, 300 nM. In RAW Cell Assay, the 3 full length versions of the antibody   “AT”405, “AT”406 and “AT”407 showed IC50 of 20.1 nM, 60.3 nM & 21.4 nM,   respectively. 

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.