Key features and details | |
Cat. No. | MABL-2549 |
Name | Anti-OPGL mAbs |
Clone No. | AFD- 18B2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, NTRL, SPR, ELISA |
Species Reactivity | Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody is specific for human OPGL. In vivo experiments have shown cross reactivity with Cynomolgus Monkey OPGL. |
Alternative Name | CD254; Tumor necrosis factor ligand superfamily member 11; TNFSF11; TNF superfamily member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; OPTB2; Receptor activator of nuclear factor kappa-B ligand; RANKL; hRANKL2; TNF-related activation-induced cytokine; TRANCE; sOdf; TNLG6B |
UniProt | O14788 |
Immunogen | The original antibody was generated by immunizing transgenic HuMab mice with purified recombinant OPGL derived from E. coli or CHO cells. Sera from immunized mice were tested for antibody binding to OPGL. |
Application Notes | The binding affinity of this antibody's original format (human IgG1) to hOPGL was confirmed by ELISA. Its binding kinetics to hOPGL 140 and hOPGL 158 was measured by SPR analysis; a KD of 0.211 nM was measured for hOPGL 140. This antibody was evaluated for its neutralizing activity against osteoclast formation induced by Osteoprotegerin ligand (OPGL) using RAW 264.7 cells, as measured by tartrate-resistant acid phosphatase (TRAP) activity; it inhibited osteoclast formation in a dose-dependent manner with an IC50 of 80 ng/ml. This antibody's pharmacokinetics and in vivo activity were assessed in cynomolgus monkeys — three female cynomolgus monkeys (not greater than five years of age, each weighing between 2 to 5 kgs) received a single subcutaneous dose of 1 mg/kg; serum samples were collected over 56 days to determine the levels of the antibody and analyze bone turnover markers such as serum N-telopeptide (serum N-Tx). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
