Key features and details | |
Cat. No. | MABL-2632 |
Name | Anti-PD-1 mAbs |
Clone No. | AFD- 1G4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | therapeutic, ELISA, FC |
Species Reactivity | Human, Rhesus Monkey |
Basic Information | |
Specificity | This antibody binds human PD-1 protein. PD-1 acts as an inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. It delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. |
Alternative Name | CD279; PD1; PD 1; hPD-1; PDCD1; Programmed cell death protein 1; Programmed cell death 1; Protein PD-1; 41-1G4; 1G4-mHvKv |
UniProt | Q15116 |
Immunogen | The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human PD-1 protein. |
Application Notes | The initial binding characterization of the antibody was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey PD-1 expressed on CHO cells was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey PD-1 and not mouse PD-1. The binding affinity of the original mouse IgG4 antibody for human PD-1 was determined using surface plasmon resonance and was found to be Kd= 1.07 nM. The anti-tumor effect of this antibody was tested in vivo in a mouse colon cancer model. Murine MC-38 cells (colon adenocarcinoma cells) were injected subcutaneously in B-hPD-1 mice and treated with this antibody. It was reported that this antibody showed good tumor inhibitory effect (CN111763257). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
