Anti-PD-1 mAbs

Cat. No.

MABL-2633

Name

Anti-PD-1 mAbs

Clone No.

AFD- 1H3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, neutralizing, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

The antibody binds specifically to PD-1.

Alternative Name

CD279; PD1; PD 1; Programmed cell death   protein 1; Programmed cell death 1; pdcd1; Protein PD-1; hPD-1

UniProt

Q15116

Immunogen

The original antibody was generated by   immunizing mice with human PD-1-Fc.

Application Notes

The original format of the antibody was able   to bind to PD-1 expressed on the surfaces of CHO cells, as measured by median   fluorescence intensity. The antibody exhibited neutralizing activity and   recognized antigen. CHO cells transfected with human full length PD-1 were   pre-incubated with the human chimeric version of the antibody and stained by   biotin-labeled anti-hB7-H1-Fc or anti-hB7-DC mFc. The antibody could bind to   the cells and block binding of B7-H1-Fc and B7-DC-Fc to cells expressing   human PD-1. The chimeric version of the antibody exhibited an affinity KD of   2.19 nM. The EC50 of the construct was found to be 75 ng. The chimeric   antibody demonstrated enhanced binding to both CD8 and CD4 cells. The   chimeric version of the antibody was found to mediate approximately a 7-fold   increase in T cell proliferation and approximately a 12 fold increase in   IFN-γ production per cell.

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.