Key features and details | |
Cat. No. | MABL-2990 |
Name | Anti-Sulphonamide drug mAbs |
Clone No. | AFD- 4D11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody shows high sensitivity for detection of sulfadoxine (SDM), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfamethazine (SMZ), and sulfaquinoxaline (SQX). It had high affinity for sulfisomidine (SIM), SMZ (including acetyl-SMZ), and sulfanitran (SNT). This antibody has higher affinity for the sulfonamides containing a six-atom-ring substitution (except sulfabenzamide -SBA and SDM) rather than a five atom-ring substitution at the N1 position for e.g. sulfamethizole (SMT) and sulfathiazole (STZ).Sulfonamides are synthetic bacteriostatic antibiotics that competitively inhibit conversion of p-aminobenzoic acid to dihydropteroate, which bacteria need for folate synthesis and ultimately purine and DNA synthesis. |
Alternative Name | SDM; SMM; SMX; SMZ; SQX; sulfadoxine; sulfamonomethoxine ; sulfamethoxazole; sulfamethazine; sulfaquinoxaline |
UniProt | |
Immunogen | The original antibody was generated by immunizing mice with a hapten made by covalently conjugating 4-(4-(4-aminophenylsulfonamido)phenyl)butanoic acid (SA10) with BSA. |
Application Notes | The binding reactivity of this antibody for various sulphonamide drugs was determined using ELISA (PMID: 23417550). The binding affinity of this antibody towards various sulphonamide drugs is listed in table 1 of PMID: 30580515. This antibody was used in the development of broad-specific and sensitive competitive indirect ELISA for screening for sulfonamides. It used MAb 4D11 and the BS-BSA as heterologous-coating antigen and demonstrated a 50 % specific binding (IC50) for 22 sulfonamides at concentrations below 100 ng mL−1. The optimized ciELISA was used to quantify the five sulfonamides, SMZ, SDM, SQX, SMM, and SMX in spiked milk samples (Lian et al., Food Anal. Methods (2014)). |
Antibody First Published | Wang et al. Monoclonal antibodies with group specificity toward sulfonamides- selection of hapten and antibody selectivity. Anal Bioanal Chem. 2013 May;405(12):4027-37. PMID:23417550 |
Note on publication | Describes the generation of antibodies with wide specificity for drugs with sulfonamide group. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
