Key features and details | |
Cat. No. | MABL-3252 |
Name | Anti-YFV-E protein mAbs |
Clone No. | AFD- 7A |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, IP, neutralize, ELISA |
Species Reactivity | Yellow Fever Virus |
Basic Information | |
Specificity | This antibody binds a conformational epitope on the envelope protein of the yellow fever virus comprising aa 153–155 in domain I and aa 71 in domain II of the E protein contributing to the epitope. |
Alternative Name | E protein; Envelope protein E; Genome polyprotein |
UniProt | P03314 |
Immunogen | The original antibody was isolated from two antibody-phage libraries constructed by repertoire cloning from yellow fever patients and subsequent panning with glycerol-purified non-inactivated YFV-17D virions. |
Application Notes | The original antibody showed 50% and 100% neutralizing activity in plaque reduction neutralization assay (PRNT) against YFV strains 17D-204-WHO and Asibi at concentrations of approximately 1 μg/ml and 10 μg/ml respectively. The original antibody neutralized wild-type YFV strains of genotypes West Africa I (Nigeria 1987) and II (Asibi) and East/Central Africa (CAR 1986, Ethiopia 1961) with comparable efficiency in a PRNT on PS cells. In a competition ELISA using biotinylated scFv-7A, this antibody fragment competed for binding to YFV-17D virions with the other neutralizing antibody fragments. The original scFv version of this antibody was able to immunoprecipitate a 55-kDa protein, corresponding to the molecular weight of the envelope glycoprotein of YFV-17D virions (PMID: 15919103). |
Antibody First Published | Daffis et al. Antibody responses against wild-type yellow fever virus and the 17D vaccine strain: characterization with human monoclonal antibody fragments and neutralization escape variants. Virology. 2005 Jul 5;337(2):262-72. PMID:15919103 |
Note on publication | Describes the generation, characterization and neutralizing capabilities of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
