Key features and details | |
Cat. No. | MABL-310 |
Name | Anti-3-pHis mAbs |
Clone No. | AFD- SC44-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IAP, WB |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for 3-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers. |
Alternative Name | 3-phosphohistidine; N3-Phosphohistidine; histidine |
UniProt | |
Immunogen | The original rabbit version of this antibody was raised by immunizing rabbits with a 3-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH). |
Application Notes | This antibody can be used for the identification of 3-pHis substrates and functional study of pHis. It was shown to detect various proteins containing 3-pHis, such as SCS and ACLY (Fuhs et al, 2015; pmid: 26140597). SC44-1 was also used for phosphoenrichment using immunoaffinity purification (Hunter et al., 2019). This clone was used to analyse the prevalence of 3-pHis in hepatocellular carcinoma by Wester blot(Hindupuretal.,2018;pmid: 29562234). In the same study, SC44-1 was further utilized to demonstrate that LHPP is a protein histidine phosphatase (Hindupur et al., 2018;pmid: 29562234). |
Antibody First Published | Fuhs et al. Monoclonal 1- and 3-Phosphohistidine AntibodiesNew Tools to Study Histidine Phosphorylation Cell. 2015 Jul 2; 162(1): 198–210. PMID:26140597 |
Note on publication | Describes the generation and characterization of the monoclonal antibodies (mAbs) that specifically recognize 1-pHis or 3-pHis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
