Anti-beta 1 integrin mAbs

Cat. No.

MABL-503

Name

Anti-beta 1 integrin mAbs

Clone No.

AFD- AIIB2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FACS, in vivo, inhibition, IP, FC

Species Reactivity

Human, Mouse

Basic Information

Specificity

The antibody specifically binds β1 integrin   extracellular domain.

Alternative Name

integrin beta 1; Integrin beta-1-binding   protein 1; Integrin cytoplasmic domain-associated protein 1 (ICAP-1)

UniProt

O14713

Immunogen

The original antibody was isolated by   immunizing a Lewis rat with JAR choriocarcinoma cells.

Application Notes

The antibody immunoprecipitated the integrin   subunits (Hall et al.,1990). This antibody was used for detection of β1   integrin expressed on CHO cells by flow cytometry (Takada et al., 1993; PMID:   7688727). The original format of the antibody or its F(ab′)2 fragments   applied to single cells were capable of down-modulating β1 integrin signaling   pathways (Wang et al., 1998; PMID: 9843973 and Weaver et al., 1997; PMID: 9105051). The antibody was used in immunofluorescence characterization of   integrins in the HMT-3522 cells (Weaver et al., 1997; PMID: 9105051). The  antibody inhibited tumor cell growth in several breast cancer cell lines   (T4-2, MDA-MB-231, BT474, SKBR3, and MCF-7) and one nonmalignant cell line   (S-1). Further, β1 integrin inhibition resulted in a significant loss of   cancer cells, associated with a decrease in proliferation and increase in   apoptosis, and a global change in the composition of residual colonies (Park   et al., 2010; PMID: 16452209). The antibody was found to reduce the frequency   of solid tumor stem cells. The antibody inhibited the growth of colon tumors  and reduced the percentage of CD44+ cells. Tumors treated in mice showed a   decrease when treated with the antibody.The humanized version of the antibody   was constructed, showing potential for therapeutic uses (WO2009009114A3).

Antibody First Published

Hall et al. The alpha 1/beta 1 and alpha 6/beta 1 integrin heterodimers mediate cell attachment to distinct sites on   laminin. J Cell Biol (1990) 110 (6): 2175–2184.

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.