Name
UFD1L Human
Cat. No.
MAG-6714
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
UFD1L Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 330 amino acids (1-307) and having a molecular mass of 36.9kDa. UFD1L is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms
Ubiquitin Fusion Degradation 1 Like (Yeast), UB Fusion Protein 1, Ubiquitin Fusion Degradation Protein 1 Homolog, UFD1.
Introduction
UFD1L forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.
Physical Appearance
Sterile filtered colorless solution.
Formulation
The UFD1L solution contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 30% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS MFSFNMF DHPIPRVFQN RFSTQYRCFS VSMLAGPNDR SDVEKGGKII MPPSALDQLS RLNITYPMLF KLTNKNSDRM THCGVLEFVA DEGICYLPHW MMQNLLLEEG GLVQVESVNL QVATYSKFQP QSPDFLDITN PKAVLENALR NFACLTTGDV IAINYNEKIY ELRVMETKPD KAVSIIECDM NVDFDAPLGY KEPERQVQHE ESTEGEADHS GYAGELGFRA FSGSGNRLDG KKKGVEPSPS PIKPGDIKRG IPNYEFKLGK ITFIRNSRPL VKKVEEDEAG GRFVAFSGEG QSLRKKGRKP
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
