Name
POLE3 Human
Cat. No.
MAG-6796
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
POLE3 Human Recombinant produced in E. coli is a single polypeptide chain containing 170 amino acids (1-147) and having a molecular mass of 19kDa. POLE3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Synonyms
CHARAC17, CHRAC17, p17, YBL1,DNA polymerase epsilon subunit 3, Arsenic-transactivated protein,AsTP, Chromatin accessibility complex 17 kDa protein, HuCHRAC17, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17, POLE3.
Introduction
Polymerase (DNA Directed), Epsilon 3, also know as POLE3, is a histone-fold protein which interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers merge into larger enzymatic complexes for DNA transcription, replication, and packaging.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The POLE3 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS MAERPED LNLPNAVITR IIKEALPDGV NISKEARSAI SRAASVFVLY ATSCANNFAM KGKRKTLNAS DVLSAMEEME FQRFVTPLKE ALEAYRREQK GKKEASEQKK KDKDKKTDSE EQDKSRDEDN DEDEERLEEE EQNEEEEVDN.
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
