Anti-COVID-19 & SARS-CoV S glycoprotein mabs [CR3022](MABL-3551)

Cat. No.

MABL-3551

Name

Anti-COVID-19 & SARS-CoV S glycoprotein  mabs

Clone No.

CR3022

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallography, NTRL, SPR, ELISA, IF

Species Reactivity

SARS-CoV-2 (COVID-19)&SARSCoronavirus

Basic Information

Specificity

This antibody binds the amino acids 318-510 in   the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope is only accessible   in the "open" confromation of the spike protein (Joyce et al.   2020).

Alternative Name

Spike protein; COVID19; COVID 19; S protein;   SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1;   SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019- nCoV; Ab1680.10; Ab1680.15;   Ab1680.16

UniProt

P59594

Immunogen

The original monoclonal antibody was generated through an scFv library derived from a peripheral blood lymphocytes   of a patient exposed to the SARS-CoV.

Application Notes

This antibody binds to both SARS-CoV and   SARS-CoV-2 with high affinity (PMID: 16796401 & 32065055). The initial  characterization of the binding of this antibody was performed by ELISA and   indicates potential for the development of diagnostic assays, as both   virus-capture assays, or as controls in serological assays measuring   immune-responses to virus exposure. Human IgG1, IgG3, IgM and IgA isotypes   are available to mimic antibody responses seen in COVID19 (Amanat et al.   2020). Human IgG2 and IgG4 subtypes, which are also seen in a small subset of   COVID-19 patients, are also available to investigate their role in the   response to SARS-CoV-2. The original human IgG1 version of the antibody works   synergistically in combination with another non-ompeting SARS antibody CR3014   and is a potential candidate for passive immune prophylaxis of SARS-CoV infection (Meulen et al., 2006). The original antibody (human IgG1) was also   reported to bind the 2019-nCoV RBD (KD of 6.3 nM). 

Antibody First   Published

ter Meulen et al. Human Monoclonal Antibody   Combination against SARS Coronavirus: Synergy and Coverage of Escape Mutants   PLoS Med. 2006 Jul; 3(7): e237 PMID:16796401

Note on publication

Describes the discovery and original characterization of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.