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Anti-Spike protein mabs [MR17](MABL-3600)

简要描述:

This antibody binds the receptor binding domain (RBD) of the SARS-CoV-2.

产品参数:

Cat. No.:MABL-3600

Species:Engineer

Species Reactivity:SARSCoronavirus2 (SARS-Cov-2)

Type:Recombinate

Application:biolayerinterferometry, FACS, NTRL, therapeutic, ELISA

详细介绍

Key features and   details


Cat. No.

MABL-3600

Name

Anti-Spike protein mabs [MR17](MABL-3600)

Clone No.

MR17

From

Recombinant Antibody

Isotype

Engineer antibody

Application

biolayerinterferometry, FACS, NTRL, therapeutic, ELISA

Species Reactivity

SARSCoronavirus2   (SARS-Cov-2)

Basic Information


Specificity

This antibody binds the receptor binding domain (RBD) of the SARS-CoV-2.

Alternative Name

SARS CoV 2 S glycoprotein; COVID-19 Spike   protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S   protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV   2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike   glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2   spike glycoprotein; Severe acute respiratory syndrome virus 2 spike   glycoprotein; Spike glycoprotein; 2019-nCoV

UniProt

P0DTC2

Immunogen

The original antibody was selected after one   round of ribosome display using three high diversity libraries (concave, loop and covex) followed by three rounds of phage display and panning against   biotinylated RBD as a bait and increasing the stringency of selection with   every round. The last round of selection was against 5nM RBD.

Application Notes

Wild type MR17 had a modest neutralization   activity. ELISA was used to screen the libraries to identify specific RBD   binders. Neutralization activity of the sybody was checked by pre- incubating   pseudoviral particles with different concentrations of sybody before   infection of VeroE6-hACE2 cells. The rate of infection was then measured by   fluorescence-activated cell sorting (FACS). IC50 value of wild type MR17 was   determined to be 12.32 µg m/L. This sybody is a K99Y mutant version of wild   typr MR17. It is seen that the single mutation increased the binding affinity   by 1.5 fold and decreased the off- rate by 2.8 fold. Overall neutralization   activity was increased 24 fold after the mutation was introduced, with an   IC50 value of 0.50 µg m/L. The binding kinetics between sybodies and the RBD   was assessed using bio-layer interferometry (Li et al., 2020).

Antibody First   Published

Li et al. Potent synthetic nanobodies against SARS-CoV-2 and molecular basis for neutralization. BioRxiv (2020)

Note on publication

Describes the generation, neutralization activity, structural analysis and mutant version of the sybody with superior   binding and neutralizing activity.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


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