Anti-Covid-19 & SARS-CoV Nucleocapsid mabs [1C7C7](MABL-3598)

Cat. No.

MABL-3598

Name

Anti-Covid-19 & SARS-CoV Nucleocapsid mabs [1C7C7](MABL-3598)

Clone No.

1C7C7

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, FC, IHC

Species Reactivity

SARS-CoV, SARS-CoV-2

Basic Information

Specificity

This antibody specifically targets an   epitope on the SARS-CoV-2 and SARS-CoV nucleocapsid protein and does not   cross-react with the nucleocapsid of other common human coronaviruses, OC43 and 229E.

Alternative Name

nucleoprotein; NP; NC; Protein N;   Nucleocapsid protein; SARS-CoV Protein N; SARS-CoV-2 Nucleocapsid protein;   SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 1C7; severe acute   respiratory syndrome virus

UniProt

P0DTC9

Immunogen

The original version of this antibody was   raised against SARS-CoV-1/2.

Application Notes

The antibody 1C7C7 detects SARS-CoV-2 and   SARS-CoV-1 and can be used in affinity binding assays, flow cytometry,   immunocytochemistry, and western blotting. Its effectiveness in detecting the   nucleocapsid of the novel coronavirus causing COVID-19 was confirmed in   multiple studies. For instance, it was used to analyze SARS-CoV-2   nucleoprotein extracted from infected cells via Western blot (Hoagland et   al., 2020) ,and similarly by Blanco-Melo et al. in a study on imbalanced host   response to SARS- CoV-2 (2020; pmid: 32416070). Another group has used this   clone to stain infected cells fixed with 10% neutral formalin during a   neutralization assay (Piepenbrink et al., 2020). Daniloski et al. performed   similar staining procedure with 1C7C7 for a trans-complementation assay   (2020; pmid: 33147445). Finally, this antibody was also used to stain   SARs-CoV-2 infected cells in a study on in vitro susceptibility to SARS-CoV-2   infection (Dobrindt et al., 2020; pmid: 32995783).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.