Anti-Covid-19 & SARS-CoV Nucleoprotein mabs [CR3009 (03-009)](MABL-3553)

Cat. No.

MABL-3553

Name

Anti-Covid-19 & SARS-CoV Nucleoprotein mabs [CR3009 (03-009)](MABL-3553)

Clone No.

CR3009(03-009)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, IF

Species Reactivity

SARS-CoV, SARS-CoV2

Basic Information

Specificity

This antibody recognizes and binds the non-linear/conformational epitope of the N protein of SARS CoV and also binds   the SARS CoV2 nucleoprotein.

Alternative Name

NP; NC; Protein N; Nucleocapsid protein; SARS-CoV Protein N; SARS-CoV Nucleocapsid protein; SARS Coronavirus;   SARS-CoV-2; SARS CoV 2; 2019-nCoV

UniProt

P0DTC9, P59595

Immunogen

The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The   harvested supernatents were then purified on protein A columns. The original antigen was the whole irradiated virion.

Application Notes

This antibody is recommended for detection   of SARS CoV2 protein N (nucleoprotein). This antibody binds both the   nucleocapsid protein of the SARS-CoV and SARS CoV-2 (2019-nCoV). Initial characterization of the antibody for binding to 2019-nCoV was done using   ELISA. This antibody shows potential to be used for development of diagnostic   assays. Various isotype versions of the antibody namely human IgG1, IgG3,   IgM, IgA and the less common IgG2 and IgG4 are available for the   investigation of their role in response to SARS CoV2. Competitive ELISA of   this antibody with CR3018 suggests that both these antibodies bind different   epitopes of the N protein of SARS CoV. Thus, a combination of these two   antibodies is suggested for virus capture assays. Immunofluorescence staining was used to demonstrate binding of CR3009 to SARS-CoV infected Vero cells.   (PMID:15650189)

Antibody First   Published

Van Den Brink et al. Molecular and Biological Characterization of Human Monoclonal Antibodies Binding to the   Spike and Nucleocapsid Proteins of Severe Acute Respiratory Syndrome Coronavirus. Journal of Virology (2005); Vol-79 (3); p. 1635–1644.   PMID:15650189

Note on publication

Describes the generation and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.