Anti-SARS-CoV S glycoprotein mabs [A11](MABL-3581)

Cat. No.

MABL-3581

Name

Anti-SARS-CoV S glycoprotein mabs 

Clone No.

A11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IF

Species Reactivity

SARSCoronavirus

Basic Information

Specificity

This antibody specifically binds the epitope in the S2 domain of the spike protein in the region of Leu803-Ala828.

Alternative Name

Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S2

UniProt

P59594

Immunogen

The original antigen was produced by subcloning the scFv gene fragment in an expression vector like pET32b, and   transforming the vector into an E.coli strain BL21 and inducing expression by   IPTG ( isopropyl-b-D-thiogalactopyranoside). A scFv phage antibody library   was constructed in fd phage. The antibody was produced by panning against   S2b, two non-immune human Ab phage display libraries and neutralizing   activity was tested against pseudotyped viruses. Antigen binding antibodies   were identified by phage ELISA.

Application Notes

This antibody binds the amino acids Leu803-Ala828 in the S2 domain of the SARS- CoV. S2 domain mediates membrane fusion between the virus and the host cell. The binding specificity of   antibody to S2b fragment of the viruse was checked by ELISA. Immunofluorescence staining was used to stain infected Vero cells using the   A11-scFv. A11-scFv recognized recombinant spike protein S2-b during western blot analysis (Leung et al., 2008).

Antibody First   Published

Leung et al. Development of human   single-chain antibodies against SARS- associated coronavirus. Intervirology.   (2008); 51(3):173-81. PMID:18724064

Note on publication

Describes the development of a single chain antibody against the S2 domain of the spike protein of the SARS coronavirus.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.