Anti-SARS-CoV-2 nucleocapsid mabs [mBG86](MABL-3593)

Cat. No.

MABL-3593

Name

Anti-SARS-CoV-2 nucleocapsid mabs

Clone No.

mBG86

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IF

Species Reactivity

SARS-CoV-2

Basic Information

Specificity

mBG86 is specific to SARS-CoV-2 N protein   and does not react with the SARS-CoV N protein or other similar N proteins (such as MERS-CoV, HuCoV-OC43, HuCoV-NL63, HuCoV-HKU1 and HuCoV-229E N).   Western blot analysis with truncated fragments of N protein suggested that   its epitope resides in AA133- 179.

Alternative Name

NP; nucleoprotein; NC; Protein N;   Nucleocapsid protein; SARS-CoV-2 Protein N; SARS-CoV-2 Nucleocapsid protein;   SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 86

UniProt

P0DTC9

Immunogen

The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a recombinant SARS-Cov-2 N   protein corresponding to AA133-419.

Application Notes

Specificty of the original mouse IgG1   version of this antibody to SARS-CoV-2 was confirmed by ELISA (Terry et al.,   2021; pmid: 33714753). mBG86 is a particularly useful antibody as it can be   used to detect SARS-Cov-2 N protein and differentiate it from SARS-CoV.   Western blot analysis demonstrated that this antibody exhibits two band   labeling, suggesting a specificity for conformational epitopes on the N   protein (Terry et al., 2021; pmid: 33714753). This antibody was shown to   detect SARS- CoV-2 N protein in methanol-stained Vero cells by   immunofluorescence (Terry et al., 2021; pmid: 33714753). Finally, the   original version of mBG86 was demonstrated to be an effective detection   antibody in a sandwich ELISA assay suggesting it's potential use in   SARS-Cov-2 diagnostics (Terry et al., 2021; pmid: 33714753).

Antibody First   Published

Terry et al. Development of SARS-CoV-2 Nucleocapsid Specific Monoclonal Antibodies Terry JS, Anderson LB, Scherman MS, McAlister CE, Perera R, Schountz T, Geiss BJ. Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody. Virology. 2021 Jun;558:28-37. PMID:33714753

Note on publication

Describes the generation and characterization of novel anti-SARS-CoV-2 N protein antibodies.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.