Name
PLGF1 Human Protein
Cat. No.
MAG-2187
Tag/Conjugates
His
Source
Escherichia coli.
Shipping
At Room Temperature
Description
PLGF1 Human Recombinant (21-221 a.a.) produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 29kDa. The PLGF-1 Human Recombinant protein is purified by proprietary chromatographic techniques.
Synonyms
PIGF, PGF, PLGF-1.
Introduction
PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
PLGF-1 protein was lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA.
Stability
Lyophilized PLGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Biological Activity
Binds to immobilized rHuVEGFR1 with EC 50 less than 6ng/ml.
Amino acid sequence
AVPPQQWALS AGNGSSEVEV VPFQEVWGRS YCRALERLVD VVSEYPSEVE HMFSPSCVSL LRCTGCCGDE NLHCVPVETA NVTMQLLKIR SGDRPSYVEL TFSQHVRCEC RPLREKMKPE RCGDAVPRR.
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
/
Solubility
It is recommended to reconstitute the lyophilized PLGF1 in sterile 18M-cm H 2 O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
