Name
PLGF1 Human Protein
Cat. No.
MAG-2189
Tag/Conjugates
His
Source
Escherichia Coli.
Shipping
At Room Temperature
Description
Placental Growth Factor-1 Human Recombinant produced in E.Coli is a non-glycosylated, disulfide-linked homodimer consisting of 2x132 amino acid polypeptide chains, having a total molecular mass of approximately 29.7kDa. PLGF1 is purified by proprietary chromatographic techniques.
Synonyms
PIGF, PGF, PLGF-1.
Introduction
PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 10mM Sodium Phosphate pH 7.5.
Stability
Lyophilized PLGF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Placental Growth Factor-1 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles .
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity
Determined by its ability to chemoattract human monocytes of using a concentration range of 1.0-10.0 ng/ml.
Amino acid sequence
MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR.
Usage
Mabioway's Co., Ltd products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
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Solubility
It is recommended to reconstitute the lyophilized Placental Growth Factor-1 in sterile 18MΩ-cm H 2 O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
