Rabbit anti-GABA (gamma-Aminobutyric Acid) Antibody

Name

Rabbit anti-GABA (gamma-Aminobutyric Acid) Antibody

中文名字

兔抗GABA抗体;兔抗γ-氨基丁酸抗体

Description

The gamma amino butyric acid antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus and cerebellum using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/15,000- 1/20,000 in PBS /0.3% Triton X-100 – biotin/avidin-HRP technique.
The specificity of the antiserum for GABA was evaluated using a competitive inhibition ELISA. While conjugates of GABA completely eliminate labeling, a 1000 fold excess of the following conjugates could not inhibit the antiserums ability to bind GABA conjugate: glutamate, aspartate, beta alanine, tyrosine, taurine, glycine and alanine.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Alligator, Armadillo, Bat, Bathynomus Doederleini (Crustacean/Isopod), Bird, Caenorhabditis Elegans Nematode (Roundworm), Cat, Drosophila (Fly), Drosophila Melanogaster (Fly), Ferret, Fish, Frog, Frog (Xenopus Laevis), Frog (Xenopus Tectum), Gerbil, Guinea Pig, Hamster, Human, Kitten (Cat), Monkey, Moth, Mouse, Parakeet, Pigeon, Pleurobranchaea Japonica (Sea Slug), Rabbit, Racoon, Rat, Slug, Snail, Turtle, Zebra Finch (Bird)

Preabsorption Control

/

Alternate Names

 anti-GABA

RRID

 AB_572234

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Rdl

Entrez Gene ID

39054

NCBI Gene Aliases

CG10537,DmRDL,DmRdl,DmelCG10537,GABA,GABA[[A]],”GABA[[A]]receptor” GABAr, LCCH1, RDL, Rd1, gaba,rd

Sequence

56-12-2

APPLICATION

Quality Control

The gamma-Aminobutyric Acid antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat thalamus and cerebellum using indirect immunofluorescent and biotin/avidin-HRP techniques. The specificity of the antiserum for GABA was evaluated using a competitive inhibition ELISA. While conjugates of GABA completely eliminate labeling, a 1000 fold excess of the following conjugates could not inhibit the antiserums ability to bind GABA conjugate: glutamate, aspartate, beta alanine, tyrosine, taurine, glycine and alanine.

Tissue

Rat thalamus and cerebellum

Perfusion Fixation

• Fixative: 4% paraformaldehyde/0.3% glutaraldehyde in 0.1M phosphate buffer, pH 7.4;500 mL 20-30 min
• Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde/0.3% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4.
• Note: Glutaraldehyde is a necessary component of fixation with this antibody. Higher concentrations of glutaraldehyde (e.g. 1–2%) may be used if needed.

Absorption Control

Sections

50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Bn/AV-HRP reagents at dilutions recommended by the manufacturer

Suggested Dilution

1/15,000–1/20,000 in PBS /0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.