Rabbit anti-GABA (gamma-Aminobutyric Acid) Antibody, diluted 1:100

Name

Rabbit anti-GABA (gamma-Aminobutyric Acid) Antibody, diluted 1:100

中文名字

兔抗GABA抗体;兔抗γ-氨基丁酸抗体,1:100稀释

Description

The antibody produces strong labeling at dilutions of 1/120 – 1/150 (which is equivalent to 1/12,000 – 1/15,000) using biotin-streptavidin/HRP technique in rat cerebellum and thalamus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
Immunolabeling is completely abolished by preadsorption with excess GABA/BSA conjugate.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Alligator, Armadillo, Bat, Bathynomus Doederleini (Crustacean/Isopod), Bird, Caenorhabditis Elegans Nematode (Roundworm), Cat, Drosophila (Fly), Drosophila Melanogaster (Fly), Ferret, Fish, Frog, Frog (Xenopus Laevis), Frog (Xenopus Tectum), Gerbil, Guinea Pig, Hamster, Human, Kitten (Cat), Monkey, Moth, Mouse, Parakeet, Pigeon, Pleurobranchaea Japonica (Sea Slug), Rabbit, Racoon, Rat, Slug, Snail, Turtle, Zebra Finch (Bird)

Preabsorption Control

/

Alternate Names

anti-GABA

RRID

AB_572233

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Entrez Gene ID

NCBI Gene Aliases

/

Sequence

56-12-2

APPLICATION

Quality Control

The antibody produces significant labeling at dilutions of 1/120 – 1/150 (equivalent to 1/12,000 - 1/15,000 in 20094) using biotin-avidin/HRP technique in rat cerebellum and thalamus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Immunolabeling is completely abolished by preabsorption with excess GABA/BSA conjugate.

Tissue

Rat thalamus and cerebellum

Perfusion Fixation

• Fixative: 4% paraformaldehyde/0.3% glutaraldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20-30 min.
• Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde/ 0.3% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4.
• Note: Glutaraldehyde is a necessary component of fixation for the GABA antiserum. Higher levels of glutaraldehyde may be used if needed.

Absorption Control

Sections

50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Bn/AV-HRP reagents at dilutions recommended by manufacturer.

Suggested Dilution

1/120–1/150 in PBS/0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry Reconstituted serum is at a dilution of 1/100. A further dilution of 120–150 times is needed to obtain the antibody dilution of 1/12,000–1/15,000 used for GABA 20094.

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.