Cat. No | MBA-025 |
Name | Rabbit anti-Gastrin Releasing Peptide (GRP) / Bombesin Antibody |
中文名字 | 兔抗胃泌素释放肽（GRP）/Bombesin抗体；兔抗GRP抗体；兔抗Bombesin抗体 |
Description | The Gastrin Releasing Peptide antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat dorsal horn of spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1,000-1/2,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of 1 mL of diluted antibody with 50 µg of bombesin. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Quantity/Volume | 50ul/100 µL |
State | Liquid Whole Serum/antibody |
Reacts With | Chick, Hamster, Human, Mouse, Newt, Pigeon, Quail, Rabbit, Ram (Sheep), Rat, Zacco Platypus (Minnow) |
Preabsorption Control | Available at irbiologicals, Catalog # IRCP069. Sequence: Glp-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH |
Alternate Names | BN; GRP-10; proGRP; preproGRP; gastrin-releasing peptide, anti-GRP, Bombesin, anti-Bombesin |
RRID | AB_572221 |
Immunogen | This information is proprietary to Mabioway |
Gene Symbol | GRP |
Entrez Gene ID | Entrez Gene: 425213 Chick |
NCBI Gene Aliases | / |
Sequence | P07492;VPLPAGGGTVLTKMYPRGNHWAVGHLM-NH2 |
APPLICATION | |
Quality Control | The Bombesin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat dorsal horn of spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Staining is completely eliminated by pretreatment of 1 mL of diluted antibody with 50 µg of bombesin. |
Tissue | Rat spinal cord dorsal horn |
Perfusion Fixation | • Fixation: 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min. |
Absorption Control | Bombesin, 50 µg/mL diluted serum. |
Sections | 10 µm cryostat |
Tissue Incubation | 18–24 hours at 2°–8°C |
Detection System | Use Cy3 or Bn/AV-HRP according to manufacturers’ directions. |
Suggested Dilution | 1/1,000–1/2,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP immunohistochemistry |
NOTES | |
Special Instructions | It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. |
Concentration | Not applicable. Antibody concentration is only relevant for purified antibodies. |
Storage | After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles. |
